Frequencies of polymorphisms in genes affecting the pharmacokinetics of warfarin in the Zaporizhzhia region

The aim of the study: to establish frequencies polymorphisms in VKORC1, CYP2C9 and CYP4F2 genes among residents Zaporizhzhia region. Materials methods. A total 150 persons (62 male, 88 female) with a median age 46 years (26; 58) undergoing preventive examination at Medical Educational Scientific Center “University Clinic” State University (ZSMU). CYP2C9, CYP4F2, VKORC1 atrial fibrillation patients were determined Department Molecular Genetic Studies ZSMU Laboratory Center. Amplification DNA fragments containing polymorphic regions was performed using multiplex real time polymerase chain reaction Warfarin Pharmacogenetics kits (LLC NPO Technology) CFX-96 thermocycler (BioRad) fluorescence detection scheme. Results. It that region residents, CYP2C9*2 genotypes were: C/C – 77.3 %, C/T 22.7 T/T 0; CYP2C9*3 genotypes: A/A 88.7 A/C 10.7 0.6 %; 56.0 35.3 8.7 G/G 38.0 G/A 50.0 12.0 %. There no statistically significant differences distribution genotype between males females different groups. populations compared. Their variability geographic established. Conclusions. are more common region, while prevalence is much lower. necessary take into account affect warfarin metabolism for each individual population select its dose by pharmacogenetic testing.